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KMID : 1098420140220060429
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Korean Journal of Medicinal Crop Science
2014 Volume.22 No. 6 p.429 ~ p.434
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A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers
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Jo Ick-Hyun
Kim Young-Chang
Kim Jang-Uk
Lee Seung-Ho
Lim Ji-Young
Moon Ji-Young
Noh Bong-Soo
Hyun Dong-Yun
Kim Dong-Hwi
Kim Ki-Hong
Bang Kyong-Hwan
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Abstract
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This study describes the efficient method for the discrimination of ‘Cheonryang’ in Panax ginseng Meyer using a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminating Korean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019, MFGp 0248, and successful identification of ‘Cheonryang’ was achieved from out of 11 Korean ginseng cultivars. Two different sizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the same size of amplified DNAs (389 bp), but ‘Cheonryang’ showed a different size. Thus ‘Cheonryang’ can be efficiently distinguished from the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizes of DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shown in five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified in the other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp 0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of ‘Cheonryang’ cultivar from a large number of samples.
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KEYWORD
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Panax ginseng Cultivar, Insertion and Deletion, Sequence Tagged Site, Genomic DNA Library
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